The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
功能Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
组织特异性Expressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
序列相似性Belongs to the amino acid/polyamine transporter 2 family.
细胞定位Cell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A1 with ab59721 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.
Anti-SLC38A1 antibody (ab59721)参考文献
This product has been referenced in:
Wang K et al. Activation of SNAT1/SLC38A1 in human breast cancer: correlation with p-Akt overexpression. BMC Cancer13:343 (2013).
Read more (PubMed: 23848995) »
Yu WL et al. Overexpression of ATA1/SLC38A1 Predicts Future Recurrence and Death in Chinese Patients with Hilar Cholangiocarcinoma. J Surg Res171:663-8 (2011).
Read more (PubMed: 20605601) »