Anti-SLC35C1抗体(ab60336)

概述

  • 产品名称
    Anti-SLC35C1抗体
    参阅全部 SLC35C1 一抗
  • 描述
    兔多克隆抗体to SLC35C1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: RALQIALVVS LYSVTSISMV FLNKYLLDSP SLRLDTPIFV TFYQCLVTTL, corresponding to N terminal amino acids 37-86 of Human SLC35C1

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab60336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

靶标

  • 相关性
    SLC35C1 is involved in GDP-fucose import from the cytoplasm into the Golgi lumen. Defects in SLC35C1 are the cause of congenital disorder of glycosylation type 2C (CDG2C), also known as leukocyte adhesion deficiency type II (LAD2). The clinical features of CDG2C include mental retardation, short stature, facial stigmata, and recurrent bacterial peripheral infections with persistently elevated peripheral leukocytes.
  • 细胞定位
    Golgi apparatus membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • FLJ11320 antibody
    • FLJ14841 antibody
    • FUCT1 antibody
    • GDP fucose transporter 1 antibody
    • Solute carrier family 35 member C1 antibody
    see all

图片

  • All lanes : Anti-SLC35C1 antibody (ab60336) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

实验方案

文献

ab60336 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing (12% Tris-Glycine)
Loading amount
30 µg
Specification
embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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提交于 Apr 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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