概述

  • 产品名称Anti-SLC29A4抗体
    参阅全部 SLC29A4 一抗
  • 描述
    小鼠单克隆抗体to SLC29A4
  • 经测试应用适用于: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment: VHHDVVAGDV HFEHPAPAPA PNESPKDSPA HEVTGSGGAY MRFDVPRPRV QRSWPTFRAL LLH, corresponding to amino acids 283-346 of Human SLC29A4

性能

应用

Our Abpromise guarantee covers the use of ab56554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性SLC29A4 (Solute carrier family 29 (nucleoside transporters), member 4) is involved in the re-uptake of monoamines into presynaptic neurons, thus determining the intensity and duration of monoamine neural signaling. It transports serotonin, dopamine and the neurotoxin 1-methyl-4-phenylpyridinium. It may also contribute to the regulation of extracellular adenosine concentrations.
  • 细胞定位Cell Membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • Brain transport protein PMAT antibody
    • ENT 4 antibody
    • ENT4 antibody
    • Equilibrative nucleoside transporter 4 antibody
    • FLJ34923 antibody
    • Plasma membrane monoamine transporter antibody
    • PMAT antibody
    • Solute carrier family 29 (nucleoside transporters) member 4 antibody
    • Solute carrier family 29 member 4 antibody
    see all

Anti-SLC29A4 antibody 图像



  • Predicted band size : 58 kDa
    SLC29A4 antibody (ab56554) at 1ug/lane + PC-12 cell lysate at 25ug/lane.
  • ICC/IF image of ab56554 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56554, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab56554 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56554, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing SH-SY5Y cells stained with ab56554 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56554, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-SLC29A4 antibody (ab56554)参考文献

This product has been referenced in:
  • André P  et al. Transport of biogenic amine neurotransmitters at the mouse blood-retina and blood-brain barriers by uptake1 and uptake2. J Cereb Blood Flow Metab : (2012). Read more (PubMed: 22850405) »

See 1 Publication for this product

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