Anti-SLC26A3抗体(ab83545)

概述

  • 产品名称Anti-SLC26A3抗体
    参阅全部 SLC26A3 一抗
  • 描述
    兔多克隆抗体to SLC26A3
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide from within the internal sequence amino acids 715 - 764: DAVLHILMKK DYSTSKFNPS QEKDGKIDFT INTNGGLRNR VYEVPVETKF, of Human SLC26A3 (NP_000102)

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab83545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Chloride/bicarbonate exchanger. Involved in absorbtion of in the colon. Helps mediate electrolyte and fluid absorption.
  • 疾病相关Defects in SLC26A3 are the cause of diarrhea type 1 (DIAR1) [MIM:214700]; also known as congenital chloride diarrhea (CLD). DIAR1 is a disease characterized by voluminous watery stools containing an excess of chloride. The children with this disease are often premature.
  • 序列相似性Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
    Contains 1 STAS domain.
  • 发展阶段Expression is significantly decreased in adenomas (polyps) and adenocarcinomas of the colon.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chloride anion exchanger antibody
    • CLD antibody
    • Congenital chloride diarrhea antibody
    • Down regulated in adenoma antibody
    • Down regulated in adenoma protein antibody
    • Down-regulated in adenoma antibody
    • DRA antibody
    • Protein DRA antibody
    • S26A3_HUMAN antibody
    • SLC26A3 antibody
    • Solute carrier family 26 member 3 antibody
    see all

Anti-SLC26A3 antibody 图像



  • Predicted band size : 85 kDa
    Western blot analysis of HepG2 cell lysate labeling SLC26A3 with ab83545 at 1.0µg/ml.
  • Anti-SLC26A3 antibody (ab83545) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa

Anti-SLC26A3 antibody (ab83545)参考文献

ab83545 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (intestine)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 50 µg
Specification intestine
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 06 2016

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