概述

  • 产品名称Anti-SLC25A28抗体
    参阅全部 SLC25A28 一抗
  • 描述
    兔多克隆抗体to SLC25A28
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: LLNTQESLAL NSHITGHITG MASAFRTVYQ VGGVTAYFRG VQARVIYQIP, corresponding to C terminal amino acids 289-338 of Human SLC25A28 (NP_112489)

  • 阳性对照
    • MCF7 cell lyaste, HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab80467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells.
  • 细胞定位Mitochondrion inner membrane; Multi pass membrane protein. Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
  • 数据库链接
  • 别名
    • MFRN2 antibody
    • Mitochondrial iron transporter 2 antibody
    • Mitochondrial RNA splicing protein 3/4 antibody
    • Mitoferrin 2 antibody
    • MRS3/4 antibody
    • MRS4L antibody
    • NPD016 antibody
    • Putative mitochondrial solute carrier antibody
    see all

Anti-SLC25A28 antibody 图像

  • Anti-SLC25A28 antibody (ab80467) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab80467 at 5 µg/ml staining SLC25A28 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ab80467 at 5 µg/ml staining SLC25A28 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ICC/IF image of ab80467 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80467, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SLC25A28 antibody (ab80467)参考文献

ab80467 has not yet been referenced specifically in any publications.

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