Anti-SLC25A20抗体(ab98227)

概述

  • 产品名称Anti-SLC25A20抗体
    参阅全部 SLC25A20 一抗
  • 描述
    兔多克隆抗体to SLC25A20
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 143-192 (SSGESKYTGT LDCAKKLYQE FGIRGIYKGT VLTLMRDVPA SGMYFMTYEW) of Human SLC25A20 (NP_000378).

  • 阳性对照
    • HT1080 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab98227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性SLC25A20 is one of several closely related mitochondrial membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. It mediates the transport of acylcarnitines into the mitochondrial matrix for their oxidation by the mitochondrial fatty acid oxidation pathway. Mutations in this gene are associated with carnitine acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
  • 细胞定位Mitochondrion inner membrane; Multipass membrane protein.
  • 数据库链接
  • 别名
    • CAC antibody
    • CACT antibody
    • Carnitine/acylcarnitine translocase antibody
    • MCAT_HUMAN antibody
    • Mitochondrial carnitine/acylcarnitine carrier protein antibody
    • Solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 antibody
    • Solute carrier family 25 member 20 antibody
    see all

Anti-SLC25A20 antibody 图像

  • Anti-SLC25A20 antibody (ab98227) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa

Anti-SLC25A20 antibody (ab98227)参考文献

ab98227 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

My colleague who routinely tests mitochondrial antibodies in WB has provided the following buffer and protocols that you may find helpful:

RIPA Buffer:

50 mM Tris-HCl pH 7.5
150 mM NaCl Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"