The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.
组织特异性High levels in heart and skeletal muscle, low in brain and very low in kidney.
疾病相关Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.
序列相似性Belongs to the mitochondrial carrier family. Contains 4 EF-hand domains. Contains 3 Solcar repeats.
Solute carrier family 25 (aspartate/glutamate carrier) member 12 antibody
Solute carrier family 25 (mitochondrial carrier Aralar) member 12 antibody
Solute carrier family 25 member 12 antibody
solute carrier family 25, member 12 antibody
Anti-SLC25A12 antibody 图像
Western blot - SLC25A12 antibody (ab90095)
Anti-SLC25A12 antibody (ab90095) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 75 kDa
Anti-SLC25A12 antibody (ab90095)参考文献
This product has been referenced in:
Povlsen JA et al. Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation. PLoS One8:e64093 (2013).
Read more (PubMed: 23704975) »
Zhong Q & Kowluru RA Diabetic retinopathy and damage to mitochondrial structure and transport machinery. Invest Ophthalmol Vis Sci52:8739-46 (2011).
Read more (PubMed: 22003103) »