概述

  • 产品名称Anti-SLC22A8抗体
    参阅全部 SLC22A8 一抗
  • 描述
    兔多克隆抗体to SLC22A8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 493-542 (PETLNQPLPE TIEDLENWSL RAKKPKQEPE VEKASQRIPL QPHGPGLGSS) of human SLC22A8 (NP_004245).

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab83789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

靶标

  • 功能Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).
  • 组织特异性Expressed in kidney.
  • 序列相似性Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
  • 细胞定位Basolateral cell membrane. Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • hOAT3 antibody
    • OAT3 antibody
    • Organic anion transporter 3 antibody
    • S22A8_HUMAN antibody
    • SLC22A8 antibody
    • Solute carrier family 22 (organic anion transporter), member 8 antibody
    • Solute carrier family 22 member 8 antibody
    see all

Anti-SLC22A8 antibody 图像

  • Anti-SLC22A8 antibody (ab83789) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-SLC22A8 antibody (ab83789)参考文献

ab83789 has not yet been referenced specifically in any publications.

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