概述

  • 产品名称
    Anti-SLC22A6抗体
    参阅全部 SLC22A6 一抗
  • 描述
    小鼠单克隆抗体to SLC22A6
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 451-550 of Human SLC22A6 with proprietary 26 kDa tag (AAH33682).

  • 阳性对照
    • Human kidney tissue
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab118346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 23516613
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 62 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

靶标

  • 功能
    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • 组织特异性
    Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • 序列相似性
    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • 结构域
    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.
  • 翻译后修饰
    Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ55736 antibody
    • hOAT1 antibody
    • hPAHT antibody
    • hROAT1 antibody
    • MGC45260 antibody
    • OAT1 antibody
    • Organic anion transporter 1 antibody
    • OTTHUMP00000236796 antibody
    • OTTHUMP00000236797 antibody
    • OTTHUMP00000236798 antibody
    • OTTHUMP00000236799 antibody
    • PAH transporter antibody
    • PAHT antibody
    • Para aminohippurate transporter antibody
    • Renal organic anion transporter 1 antibody
    • ROAT1 antibody
    • S22A6_HUMAN antibody
    • SLC22A6 antibody
    • Solute carrier family 22 (organic anion transporter) member 6 antibody
    • Solute carrier family 22 member 6 antibody
    see all

图片

  • Anti-SLC22A6 antibody (ab118346) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 62 kDa
  • ab118346, at 3 µg/ml staining SLC22A6 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • Detection limit for ab118346 is 3 ng/ml as a capture antibody

文献

This product has been referenced in:
  • Wu X  et al. Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy. Mol Med Rep 13:2385-92 (2016). Read more (PubMed: 26846716) »
  • Topalis D  et al. Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget 7:10386-401 (2016). WB . Read more (PubMed: 26824416) »

See all 3 Publications for this product

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