Recombinant fragment, corresponding to a region within amino acids 1-139 of Human SLC22A18AS (AAH30237).
A431 whole cell lysates. Gastric carcinoma tissue. 293T cell line.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.
Most abundantly expressed in gastrointestinal tissues. Expressed at lower levels in kidney and placenta. Expressed in fetal brain, liver, placenta, kidney and lung.
Information by UniProt
Beckwith-Wiedemann region 1B antibody
Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein antibody
Beckwith-Wiedemann syndrome chromosome region 1, candidate b antibody
Western blot - SLC22A18AS antibody (ab111913)
Anti-SLC22A18AS antibody (ab111913) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 27 kDa 15% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SLC22A18AS antibody (ab111913)
ab111913 at 1/500 dilution staining SLC22A18AS in paraffin-embedded gastric carcinoma tissue by Immunohistochemistry
has not yet been referenced specifically in any publications.
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