Anti-SLC22A1抗体(ab55916)

概述

  • 产品名称Anti-SLC22A1抗体
    参阅全部 SLC22A1 一抗
  • 描述
    兔多克隆抗体to SLC22A1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: LATNRSHLPL GPCQDGWVYD TPGSSIVTEF NLVCADSWKL DLFQSCLNAG, corresponding to N terminal amino acids 109-158 of Human SLC22A1

  • 阳性对照
    • Jurkat cell lysate Human intestine: epithelial cells of intestinal villus

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab55916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).Can be blocked with Human SLC22A1 peptide (ab111655). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.
  • 组织特异性Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines.
  • 序列相似性Belongs to the major facilitator superfamily. Organic cation transporter family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Basolateral cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hOCT1 antibody
    • OCT1 antibody
    • oct1_cds antibody
    • Organic cation transporter 1 antibody
    • S22A1_HUMAN antibody
    • Slc22a1 antibody
    • solute carrier family 22 (organic cation transporter), member 1 antibody
    • Solute carrier family 22 member 1 antibody
    see all

Anti-SLC22A1 antibody 图像

  • Anti-SLC22A1 antibody (ab55916) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Intestine tissue labeling SLC22A1 with ab55916 at 8µg/ml. Positively labelled epithelial cells of intestinal villus are indicated with arrows. Magnification: 400X

  • All lanes : Anti-SLC22A1 antibody (ab55916) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 8 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 61 kDa

Anti-SLC22A1 antibody (ab55916)参考文献

ab55916 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse kidneys)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: vector antigen unmasking solution H-3300
Permeabilization No
Specification mouse kidneys
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Aug 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 1%BSA and 2%NGS as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
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Verified customer

提交于 May 28 2014

Thank you for your inquiry and your patience with this reply.
I heard back from the lab regarding ab55916. The antibody is a short 14 a.a. peptide located in this sequence: LATNRSHLPL GPCQDGWVYD TPGSSIVTEF NLVCADSWKL DLFQSCLNAG. This sequence is f...

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Thank you for your reply.
Your credit note ID is ***
I am sorry that this antibody did not perform as stated on the datasheet, and I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the fo...

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Thank you your reply and for keeping me updated. I am sorry to hear that the results with ab105049 are still not as expected.
I would be happy to send a new vial of this antibody, however we only have the same batch in stock. Would you like to try...

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Thank you for your reply and for sending the additional information and the images.
For antigen retrieval prior to using ab55916, the lab microwaved the tissue in pH6 0.01 M citrate buffer for 10 minutes at ˜100C, and then the section were cooled ...

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Thank you for contacting us and for letting us know about the trouble with these antibodies in IHC-P.
Thank you also for sending the protocol information. I do have a couple of additional questions in order to better understand the situation:
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