Anti-SLC20A2抗体(ab64412)

概述

  • 产品名称Anti-SLC20A2抗体
    参阅全部 SLC20A2 一抗
  • 描述
    兔多克隆抗体to SLC20A2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: WQLIASFLRL PISGTHCIVG STIGFSLVAI GTKGVQWMEL VKIVASWFIS, corresponding to amino acids 101-150 of Human SLC20A2

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab64412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gibbon ape leukemia virus receptor 2 antibody
    • Gibbon leukemia virus receptor 2 antibody
    • GLVR 2 antibody
    • GLVR-2 antibody
    • GLVR2 antibody
    • hPit2 antibody
    • IBGC3 antibody
    • MLVAR antibody
    • Murine leukemia virus amphotropic receptor antibody
    • murine leukemia virus, amphotropic, receptor for antibody
    • Phosphate transporter 2 antibody
    • PIT 2 antibody
    • PiT-2 antibody
    • Pit2 antibody
    • RAM1 antibody
    • S20A2_HUMAN antibody
    • SLC20A2 antibody
    • Sodium-dependent phosphate transporter 2 antibody
    • Solute carrier family 20 (phosphate transporter) member 2 antibody
    • solute carrier family 20 (phosphate transporter), member 2 antibody
    • Solute carrier family 20 member 2 antibody
    see all

Anti-SLC20A2 antibody 图像

  • Anti-SLC20A2 antibody (ab64412) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-SLC20A2 antibody (ab64412)参考文献

ab64412 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (Ovary)
Permeabilization No
Specification Ovary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
Username

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Verified customer

提交于 May 29 2015

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Cell lysate - whole cell (aortic valve interstitial cells)
Specification aortic valve interstitial cells
Treatment Nah2po4 3.5 mM
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"