概述

  • 产品名称Anti-SLC19A1抗体
    参阅全部 SLC19A1 一抗
  • 描述
    兔多克隆抗体to SLC19A1
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    A 14-22 amino acid region within synthetic peptide: MVPSSPAVEK QVPVEPGPDP ELRSWRHLVC YLCFYGFMAQ IRPGESFITP, corresponding to N terminal amino acids 1-50 of Human SLC19A1

  • 阳性对照
    • Jurkat cell lysate. Human kidney.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump.
  • 组织特异性Placenta, liver, and to a much smaller extent, in lung.
  • 序列相似性Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.
  • 翻译后修饰Heavily glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHMD antibody
    • FLOT 1 antibody
    • FLOT1 antibody
    • Folate transporter 1 antibody
    • FOLT antibody
    • IFC 1 antibody
    • IFC-1 antibody
    • IFC1 antibody
    • Intestinal folate carrier 1 antibody
    • Intestinal folate carrier antibody
    • OTTHUMP00000115459 antibody
    • OTTHUMP00000115460 antibody
    • Placental folate transporter antibody
    • Reduced folate carrier antibody
    • Reduced folate carrier protein antibody
    • REFC antibody
    • RFC 1 antibody
    • RFC antibody
    • RFC1 antibody
    • S19A1_HUMAN antibody
    • SLC19A1 antibody
    • Solute carrier family 19 member 1 antibody
    see all

Anti-SLC19A1 antibody 图像

  • SLC19A1 antibody (ab62302) at 5.0µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Gel concentration: 8%
  • ICC/IF image of ab62302 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62302, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC19A1 with ab62302 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.

Anti-SLC19A1 antibody (ab62302)参考文献

This product has been referenced in:
  • Stewart DJ  et al. Decitabine impact on the endocytosis regulator RhoA, the folate carriers RFC1 and FOLR1, and the glucose transporter GLUT4 in human tumors. Clin Epigenetics 6:2 (2014). Read more (PubMed: 24401732) »
  • Halwachs S  et al. The antiepileptic drugs phenobarbital and carbamazepine reduce transport of methotrexate in rat choroid plexus by down-regulation of the reduced folate carrier. Mol Pharmacol 80:621-9 (2011). WB ; Rat . Read more (PubMed: 21737571) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Rat Tissue lysate - other (Brain)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 30 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Username

Ms. Aniv Mann

Verified customer

提交于 Oct 30 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (Polyacrylamide gel precast 12-4%)
Sample Human Cell lysate - whole cell (THLE2 liver cell line)
Specification THLE2 liver cell line
Blocking step Li-cor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 04 2014

Thank you for your message,

I apologize for any confusion. We do sell ab62302 Anti-SLC19A1 antibodyin lyophillised form now. I can suggest in this case youhave receivedan older vial which has been reconstituted already.This can be used as no...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.
The details your customer has kindly provided will...

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Application Western blot
Sample Rat Cell lysate - whole cell (choroidal epithelial Z310 cells)
Loading amount 50 µg
Specification choroidal epithelial Z310 cells
Gel Running Conditions Reduced Non-Denaturing (Native) (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"