概述

  • 产品名称Anti-SLC15A2抗体
    参阅全部 SLC15A2 一抗
  • 描述
    兔多克隆抗体to SLC15A2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 432-481 (GNENNSLLIE SIKSFQKTPH YSKLHLKTKS QDFHFHLKYH NLSLYTEHSV) of human SLC15A2 (NP_066568).

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides.
  • 序列相似性Belongs to the PTR2/POT transporter (TC 2.A.17) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ33407 antibody
    • Hydrogen ion/peptide cotransporter, kidney antibody
    • Kidney H(+)/peptide cotransporter antibody
    • kidney isoform antibody
    • Oligopeptide transporter antibody
    • Oligopeptide transporter kidney isoform antibody
    • PEPT2 antibody
    • Peptide transporter 2 antibody
    • S15A2_HUMAN antibody
    • Slc15a2 antibody
    • Solute carrier family 15 (H+/peptide transporter) member 2 antibody
    • solute carrier family 15 (oligopeptide transporter), member 2 antibody
    • Solute carrier family 15 member 2 antibody
    see all

Anti-SLC15A2 antibody 图像

  • Anti-SLC15A2 antibody (ab83771) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83771 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83771, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SLC15A2 antibody (ab83771)参考文献

ab83771 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83771.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"