概述

  • 产品名称Anti-SLC12A1抗体
    参阅全部 SLC12A1 一抗
  • 描述
    兔多克隆抗体to SLC12A1
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, African Green Monkey
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Jurkat cells; Kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab60301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

  • 功能Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • 组织特异性Kidney specific.
  • 疾病相关Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • 序列相似性Belongs to the SLC12A transporter family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSC1 antibody
    • Bumetanide sensitive sodium 3 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
    • Kidney specific Na K Cl symporter antibody
    • Kidney-specific Na-K-Cl symporter antibody
    • MGC48843 antibody
    • Na K 2Cl cotransporter antibody
    • NKCC2 antibody
    • potassiumchloride cotransporter 2 antibody
    • S12A1_HUMAN antibody
    • Slc12a1 antibody
    • sodium potassium chloride cotransporter 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
    • Solute carrier family 12 member 1 antibody
    see all

Anti-SLC12A1 antibody 图像

  • All lanes : Anti-SLC12A1 antibody (ab60301) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Immunohistchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC12A1 with ab60301 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-SLC12A1 antibody (ab60301)参考文献

ab60301 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab60301.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"