antigen, corresponding to amino acids 794-898 of Human SKT (UniProt ID: Q5T5P2).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Required for normal development of intervertebral disks.
Information by UniProt
- DKFZP761L0424 antibody
- KIAA1217 antibody
- Likely orthologue of Mus musculus enhancer trap locus 4 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SKT antibody (ab121223)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121223 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKT antibody (ab121223)
ab121223, at 1/40 dilution, staining SKT in paraffin embedded Human duodenum tissue by Imunohistochemistry.
has not yet been referenced specifically in any publications.
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