RabMAb

Anti-SKP2抗体[EPR3306(2)] (ab124799)

概述

  • 产品名称Anti-SKP2抗体[EPR3306(2)]
    参阅全部 SKP2 一抗
  • 描述
    兔单克隆抗体[EPR3306(2)] to SKP2
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues in Human SKP2.

  • 阳性对照
    • 293T, HeLa, SW480 and LnCaP cell lysates; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 1 F-box domain.
      Contains 9 LRR (leucine-rich) repeats.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDK2/Cyclin A associated protein p45 antibody
      • Cyclin A/CDK2 associated protein p45 antibody
      • Cyclin-A/CDK2-associated protein p45 antibody
      • F box protein Skp2 antibody
      • F box/LRR repeat protein 1 antibody
      • F-box protein Skp2 antibody
      • F-box/LRR-repeat protein 1 antibody
      • FBL 1 antibody
      • FBL1 antibody
      • FBXL 1 antibody
      • FBXL1 antibody
      • FLB 1 antibody
      • FLB1 antibody
      • MGC1366 antibody
      • p45 antibody
      • p45skp2 antibody
      • S phase kinase associated protein 2 (p45) antibody
      • S phase kinase associated protein 2 antibody
      • S-phase kinase-associated protein 2 antibody
      • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
      • SKP 2 antibody
      • Skp2 antibody
      • SKP2_HUMAN antibody
      see all

    Anti-SKP2 antibody [EPR3306(2)] 图像

    • All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : LnCaP cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46, 48 kDa
    • ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence.

    Anti-SKP2 antibody [EPR3306(2)] (ab124799)参考文献

    ab124799 has not yet been referenced specifically in any publications.

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