Anti-SKP2抗体(ab68455)
Key features and details
- Rabbit polyclonal to SKP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SKP2抗体
参阅全部 SKP2 一抗 -
描述
兔多克隆抗体to SKP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SKP2 aa 2-20 (N terminal). Different from the related rat and mouse sequences by three amino acids.
Sequence:HRKHLQEIPDLSSNVATSF
Database link: Q13309 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 6502 Human
- Omim: 601436 Human
- SwissProt: Q13309 Human
- Unigene: 23348 Human
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别名
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
see all
图片
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All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Hela cells
Lane 2 : Whole cell lysates prepared from 6T-CEM cells
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 48 kDa -
ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
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All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The predicted molecular weight of SKP2 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 53 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab68455 被引用在 15 文献中.
- Liu B et al. SKP2-Promoted Ubiquitination of FOXO3 Promotes the Development of Asthma. J Immunol 206:2366-2375 (2021). PubMed: 33837090
- Zhang X et al. IMP3 accelerates the progression of prostate cancer through inhibiting PTEN expression in a SMURF1-dependent way. J Exp Clin Cancer Res 39:190 (2020). PubMed: 32938489
- Qiu C et al. Bisdemethoxycurcumin Inhibits Hepatocellular Carcinoma Proliferation Through Akt Inactivation via CYLD-Mediated Deubiquitination. Drug Des Devel Ther 14:993-1001 (2020). PubMed: 32184568
- Yang Y et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res 52:e8412 (2019). PubMed: 31038581
- Yan W et al. Inhibition of SKP2 Activity Impaired ATM-Mediated DNA Repair and Enhanced Sensitivity of Cisplatin-Resistant Mantle Cell Lymphoma Cells. Cancer Biother Radiopharm N/A:N/A (2019). PubMed: 31025879