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Our Abpromise guarantee covers the use of ab68455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|ICC||Use a concentration of 1 - 2 µg/ml. Acetone fixed cells. Antigen retrieval by Pepsin and Trypsin is required.|
ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.