概述

  • 产品名称Anti-SKP2抗体
    参阅全部 SKP2 一抗
  • 描述
    兔多克隆抗体to SKP2
  • 经测试应用适用于: IP, WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence at the N terminus of human SKP2, differing from the related rat and mouse sequence by three amino acids.

性能

应用

Our Abpromise guarantee covers the use of ab68455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml.
ICC Use a concentration of 1 - 2 µg/ml. Acetone fixed cells. Antigen retrieval by Pepsin and Trypsin is required.

靶标

  • 功能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDK2/Cyclin A associated protein p45 antibody
    • Cyclin A/CDK2 associated protein p45 antibody
    • Cyclin-A/CDK2-associated protein p45 antibody
    • F box protein Skp2 antibody
    • F box/LRR repeat protein 1 antibody
    • F-box protein Skp2 antibody
    • F-box/LRR-repeat protein 1 antibody
    • FBL 1 antibody
    • FBL1 antibody
    • FBXL 1 antibody
    • FBXL1 antibody
    • FLB 1 antibody
    • FLB1 antibody
    • MGC1366 antibody
    • p45 antibody
    • p45skp2 antibody
    • S phase kinase associated protein 2 (p45) antibody
    • S phase kinase associated protein 2 antibody
    • S-phase kinase-associated protein 2 antibody
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
    • SKP 2 antibody
    • Skp2 antibody
    • SKP2_HUMAN antibody
    see all

Anti-SKP2 antibody 图像

  • ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml

    Lane 1 : Whole cell lysates prepared from Hela cells
    Lane 2 : Whole cell lysates prepared from 6T-CEM cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 48 kDa
  • All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
    The predicted molecular weight of SKP2 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 53 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • SKP2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SKP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68455.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 53kDa; SKP2

Anti-SKP2 antibody (ab68455)参考文献

This product has been referenced in:
  • Sanchez N  et al. MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. PLoS One 8:e65671 (2013). WB ; Human . Read more (PubMed: 23762407) »
  • Zhao C  et al. Diagnostic and biological significance of microRNA-192 in pancreatic ductal adenocarcinoma. Oncol Rep 30:276-84 (2013). WB ; Human . Read more (PubMed: 23612862) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (Amersham ECL Gel 8-16%)
Sample Chinese Hamster Cell lysate - whole cell (CHO DG44 cell line)
Specification CHO DG44 cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2014

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab19877.

To check the status of the order please contact o...

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Thank you for your reply.

We do not have small test samples available to send.

I would say both ab19877 and ab68887 have an equal likelihood of reacting with murine samples as both have similar percentage identity to the immunogens ...

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Thank you for your enquiry and for including the western blotting data.

I am sorry to see this vial of ab68455 is not working as expected. I would be happy to send you another vial of a different anti-SKP2 antibody. At this time none of our o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"