概述

  • 产品名称Anti-Skp1抗体[EPR3303]
    参阅全部 Skp1 一抗
  • 描述
    兔单克隆抗体[EPR3303] to Skp1
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from Human Skp1

  • 阳性对照
    • WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; FC: permeabilized HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab80586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Flow Cyt
IHC-P
  • 应用说明Flow Cyt: 1/50.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    Is unsuitable for ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the SKP1 family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cyclin A/CDK2 associated p19 antibody
      • Cyclin A/CDK2 associated protein p19 antibody
      • Cyclin-A/CDK2-associated protein p19 antibody
      • EMC19 antibody
      • MGC34403 antibody
      • OCP 2 antibody
      • OCP II antibody
      • OCP II protein antibody
      • OCP-2 antibody
      • OCP-II antibody
      • OCP2 antibody
      • OCPII antibody
      • Organ of Corti protein 2 antibody
      • Organ of Corti protein II antibody
      • OTTHUMP00000159379 antibody
      • OTTHUMP00000159380 antibody
      • OTTHUMP00000228275 antibody
      • OTTHUMP00000228276 antibody
      • OTTHUMP00000228278 antibody
      • OTTHUMP00000228279 antibody
      • OTTHUMP00000228281 antibody
      • p19A antibody
      • p19skp1 antibody
      • RNA polymerase II elongation factor like protein antibody
      • RNA polymerase II elongation factor like protein OCP2 antibody
      • RNA polymerase II elongation factor-like protein antibody
      • S phase kinase associated protein 1 antibody
      • S phase kinase associated protein 1A antibody
      • S phase kinase associated protein 1A p19A antibody
      • S-phase kinase-associated protein 1 antibody
      • SIII antibody
      • Skp 1 antibody
      • SKP 1A antibody
      • skp1 antibody
      • SKP1_HUMAN antibody
      • SKP1A antibody
      • TCEB1L antibody
      • Transcription Elongation Factor B 1 like antibody
      • Transcription elongation factor B antibody
      • Transcription elongation factor B SIII polypeptide 1 like antibody
      see all

    Anti-Skp1 antibody [EPR3303] 图像

    • All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 19 kDa
    • Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.
    • ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.

    Anti-Skp1 antibody [EPR3303] (ab80586)参考文献

    This product has been referenced in:
    • Wang D  et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 109:9493-8 (2012). WB ; Mouse . Read more (PubMed: 22645357) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Jul 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"