Anti-MTR4抗体(ab70552)
Key features and details
- Rabbit polyclonal to MTR4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MTR4抗体
参阅全部 MTR4 一抗 -
描述
兔多克隆抗体to MTR4 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human MTR4 aa 650-750.
Database link: P42285 -
阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70552于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP | (1) |
Use at 5 µg/mg of lysate.
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说明 |
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IP
Use at 5 µg/mg of lysate. |
靶标
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相关性
MTR4, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. MTR4 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA. -
细胞定位
Nucleus, nucleolus. -
数据库链接
- Entrez Gene: 23517 Human
- Entrez Gene: 72198 Mouse
- SwissProt: P42285 Human
- SwissProt: Q9CZU3 Mouse
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别名
- 2610528A15Rik antibody
- ATP dependent helicase SKIV2L2 antibody
- Dob1 antibody
see all
图片
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Detection of MTR4 by western blot of immunoprecipitates.
Samples: Lane 1: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer. Lane 2: Control IgG.
Antibodies: ab70552 used for IP at 6 µg per reaction. For blotting immunoprecipitated MTR4, ab70552 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70552 被引用在 3 文献中.
- Domanski M & LaCava J Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells. Bio Protoc 7:N/A (2017). PubMed: 28691041
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244
- Onderak AM & Anderson JT Loss of the RNA helicase SKIV2L2 impairs mitotic progression and replication-dependent histone mRNA turnover in murine cell lines. RNA 23:910-926 (2017). PubMed: 28351885