概述

  • 产品名称Anti-SKIV2L2抗体
    参阅全部 SKIV2L2 一抗
  • 描述
    兔多克隆抗体to SKIV2L2
  • 经测试应用适用于: IHC-P, ICC, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region between residues 100 and 150 of human SKIV2L2. (NP_056175.2)

  • 阳性对照
    • HeLa, and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.

Epitope retrieval with Tris-EDTA pH9.0 is recommended for formalin-fixed paraffin-embedded cells.

WB 1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性SKIV2L2, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. SKIV2L2 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA.
    • 细胞定位Nucleus, nucleolus.
    • 数据库链接
    • 别名
      • 2610528A15Rik antibody
      • ATP dependent helicase SKIV2L2 antibody
      • Dob1 antibody
      • KIAA0052 antibody
      • MGC142069 antibody
      • mKIAA0052 antibody
      • Mtr4 antibody
      • superkiller viralicidic activity 2 like 2 (S. cerevisiae) antibody
      • Superkiller viralicidic activity 2 like 2 antibody
      see all

    Anti-SKIV2L2 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling SKIV2L2 with ab70551 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-SKIV2L2 antibody (ab70551) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 118 kDa
      Observed band size : 118 kDa


      Exposure time : 10 seconds
    • IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-SKIV2L2 antibody (ab70551)参考文献

    This product has been referenced in:
    • Ebmeier CC & Taatjes DJ Activator-Mediator binding regulates Mediator-cofactor interactions. Proc Natl Acad Sci U S A 107:11283-8 (2010). WB ; Human . Read more (PubMed: 20534441) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS cells)
    Loading amount 50 µg
    Specification U2OS cells
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"