兔多克隆抗体to Skeletrophin / Mib2
Synthetic peptide, corresponding to a region between residue 925 and 975 of Human Skeletrophin/ Mib2 (NP_543151.1).
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7 to 8
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
Use at 2-5 µg/mg of lysate.
Skeletrophin, also known as Mib2, is a homolog of the zebrafish protein Mindbomb. Skeletrophin is an E3 ubiquitin ligase, expressed mainly in skeletal muscle, which is downregulated in malignant melanoma.
E3 ubiquitin-protein ligase MIB2 antibody
Mindbomb homolog 2 antibody
Western blot - Anti-Skeletrophin / Mib2 antibody (ab99378)
All lanes : Anti-Skeletrophin / Mib2 antibody (ab99378) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 110 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-Skeletrophin / Mib2 antibody (ab99378)
Lane 1 : Detection of Human Skeletrophin / Mib2 by Imunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99378 at 3µg/mg lysate.
Lane 2 : Control IgG
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"