Anti-Skeletrophin / Mib2抗体(ab99378)

概述

  • 产品名称Anti-Skeletrophin / Mib2抗体
    参阅全部 Skeletrophin / Mib2 一抗
  • 描述
    兔多克隆抗体to Skeletrophin / Mib2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 925 and 975 of Human Skeletrophin/ Mib2 (NP_543151.1).

  • 阳性对照
    • HeLa, 293T lysates
  • 常规说明Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

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应用

Our Abpromise guarantee covers the use of ab99378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性Skeletrophin, also known as Mib2, is a homolog of the zebrafish protein Mindbomb. Skeletrophin is an E3 ubiquitin ligase, expressed mainly in skeletal muscle, which is downregulated in malignant melanoma.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase MIB2 antibody
    • Mib2 antibody
    • Mindbomb homolog 2 antibody
    • Novel zinc finger protein antibody
    • Novelzin antibody
    • Putative NF-kappa-B-activating protein 002N antibody
    • SKD antibody
    • Skeletrophin antibody
    • Zinc finger ZZ type with ankyrin repeat domain protein 1 antibody
    • ZZANK1 antibody
    see all

Anti-Skeletrophin / Mib2 antibody 图像

  • All lanes : Anti-Skeletrophin / Mib2 antibody (ab99378) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 110 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human Skeletrophin / Mib2 by Imunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99378 at 3µg/mg lysate. Lane 2 : Control IgG

Anti-Skeletrophin / Mib2 antibody (ab99378)参考文献

ab99378 has not yet been referenced specifically in any publications.

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