The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at a concentration of 5 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects an ~ 60 kDa protein from Human Jurkat cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
Belongs to the SKAP family. Contains 1 PH domain. Contains 1 SH3 domain.
The SH3 domain interacts with FYB.
Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.