Anti-SKAP55抗体(ab19377)

概述

  • 产品名称Anti-SKAP55抗体
    参阅全部 SKAP55 一抗
  • 描述
    兔多克隆抗体to SKAP55
  • 特异性Detects Human SKAP55.
  • 经测试应用适用于: ICC, IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Immunogen corresponds to amino acids 2-105 of Human SKAP55:

    QAAALPEEIRWLLEDAEEFLAEGLRNENLSAVARDHRDHILRGFQQIKAR YYWDFQPQG

    .

  • 阳性对照
    • Human Jurkat cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab19377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
IP
WB
  • 应用说明ICC: Use at a concentration of 5 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Detects an ~ 60 kDa protein from Human Jurkat cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
    • 组织特异性Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
    • 序列相似性Belongs to the SKAP family.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • 结构域The SH3 domain interacts with FYB.
    • 翻译后修饰Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
    • 细胞定位Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • pp55 antibody
      • SCAP 1 antibody
      • SCAP1 antibody
      • SKAP 1 antibody
      • SKAP 55 antibody
      • SKAP-55 antibody
      • Skap1 antibody
      • SKAP1_HUMAN antibody
      • SKAP55 adaptor protein antibody
      • SRC family associated phosphoprotein 1 antibody
      • Src family-associated phosphoprotein 1 antibody
      • Src kinase associated phosphoprotein 1 antibody
      • SRC kinase associated phosphoprotein 55 kD antibody
      • Src kinase associated phosphoprotein of 55 kDa antibody
      • Src kinase-associated phosphoprotein 1 antibody
      • Src kinase-associated phosphoprotein of 55 kDa antibody
      see all

    Anti-SKAP55 antibody 图像

    • All lanes : Anti-SKAP55 antibody (ab19377) at 1/500 dilution

      Lane 1 : Human T cell lysate
      Lane 2 : Human platelet lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG conjugated to Odyssey IR-Dye 800 at 1/50000 dilution

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 51 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa (possible non-specific binding).

      Exposure time : 3 minutes

      This image is courtesy of an anonymous abreview.

      Detection: Licor Odyssey system

       

      Blocking: 3% BSA

      See Abreview

    Anti-SKAP55 antibody (ab19377)参考文献

    ab19377 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (T cell, platelet)
    Loading amount 20 µg
    Specification T cell, platelet
    Gel Running Conditions Reduced Denaturing (10 %)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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    提交于 Apr 29 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"