概述

  • 产品名称
  • 描述
    兔多克隆抗体to SKA2
  • 特异性
    This antibody is specific for SKA2 and will not crossreact with SKA1 or SKA3.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 15 amino acid peptide from near the N terminus of Human SKA2 (NP_872426).

  • 阳性对照
    • 3T3 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity chromatography purified via peptide column
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 14 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
    • 序列相似性
      Belongs to the SKA2 family.
    • 细胞定位
      Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FAM33A antibody
      • Family with sequence similarity 33, member A antibody
      • FLJ12758 antibody
      • MGC110975 antibody
      • Protein FAM33A antibody
      • SKA 2 antibody
      • SKA2 antibody
      • SKA2_HUMAN antibody
      • Spindle and kinetochore associated complex subunit 2 antibody
      • Spindle and kinetochore associated protein 2 antibody
      • Spindle and kinetochore-associated protein 2 antibody
      • Spindle and KT (kinetochore) associated 2 antibody
      • Spindle and KT associated 2 antibody
      see all

    图片

    • Lane 1 : Anti-SKA2 antibody (ab91551) at 0.5 µg/ml
      Lane 2 : Anti-SKA2 antibody (ab91551) at 1 µg/ml

      Lane 1 : 3T3 cell lysate
      Lane 2 : 3T3 cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 14 kDa

    实验方案

    文献

    This product has been referenced in:
    • Thomas GE  et al. EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice. Nat Commun 7:11665 (2016). Read more (PubMed: 27225956) »

    See 1 Publication for this product

    客户评价及客户问答

    Thanks for your call today and for letting us know about the trouble with ab91551.

    As we discussed, I'm sending a free of chargevial of ab113067 on the order ***, which should arrive early next week. Please keep me updated about how this anti...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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