Anti-SKA2抗体(ab113067)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SKA2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 16-45 of Human SKA2 (NP_872426.1).

  • 阳性对照
    • T47D cell line lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab113067 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 14 kDa.

靶标

  • 功能
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
  • 序列相似性
    Belongs to the SKA2 family.
  • 细胞定位
    Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FAM33A antibody
    • Family with sequence similarity 33, member A antibody
    • FLJ12758 antibody
    • MGC110975 antibody
    • Protein FAM33A antibody
    • SKA 2 antibody
    • SKA2 antibody
    • SKA2_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 2 antibody
    • Spindle and kinetochore associated protein 2 antibody
    • Spindle and kinetochore-associated protein 2 antibody
    • Spindle and KT (kinetochore) associated 2 antibody
    • Spindle and KT associated 2 antibody
    see all

图片

  • Anti-SKA2 antibody (ab113067) at 1/100 dilution + T47D cell line lysate at 35 µg

    Predicted band size : 14 kDa

实验方案

文献

ab113067 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for calling Abcam earlier today.

I am sorry about the problems you are having with ab113067 in western blot. As we discussed on the phone, I am sending ab14899, T47D cell lysate for you to use as a positive control. Your order numbe...

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