概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab46826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
  • 序列相似性Belongs to the SKA1 family.
  • 细胞定位Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810433K01Rik antibody
    • AV117428 antibody
    • C18orf24 antibody
    • MGC10200 antibody
    • Ska1 antibody
    • SKA1_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 1 antibody
    • Spindle and kinetochore associated protein 1 antibody
    • Spindle and kinetochore-associated protein 1 antibody
    • Spindle and KT (kinetochore) associated 1 antibody
    • Spindle and KT associated 1 antibody
    see all

Anti-SKA1 antibody (ab46826)参考文献

ab46826 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Apr 14 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 23°C
Username

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Verified customer

提交于 Feb 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"