概述

  • 产品名称Anti-SKA1抗体
    参阅全部 SKA1 一抗
  • 描述
    兔多克隆抗体to SKA1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, derived from the C-terminus of Human SKA1.

  • 阳性对照
    • A549 cell lysate; Human Brain, Cerebellum and Cortex tissues

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 29 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
  • 序列相似性Belongs to the SKA1 family.
  • 细胞定位Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810433K01Rik antibody
    • AV117428 antibody
    • C18orf24 antibody
    • MGC10200 antibody
    • Ska1 antibody
    • SKA1_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 1 antibody
    • Spindle and kinetochore associated protein 1 antibody
    • Spindle and kinetochore-associated protein 1 antibody
    • Spindle and KT (kinetochore) associated 1 antibody
    • Spindle and KT associated 1 antibody
    see all

Anti-SKA1 antibody 图像

  • Lane 1 : Anti-SKA1 antibody (ab118586) at 0.5 µg/ml
    Lane 2 : Anti-SKA1 antibody (ab118586) at 1 µg/ml

    Lane 1 : A549 cell lysate
    Lane 2 : A549 cell lysate


    Predicted band size : 29 kDa
  • ab118586, at 5 µg/ml, staining SKA1 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum tissue by Immunohistochemistry.
  • ab118586, at 5 µg/ml, staining SKA1 in formalin-fixed, paraffin-embedded Human Brain, Cortex tissue by Immunohistochemistry.

Anti-SKA1 antibody (ab118586)参考文献

ab118586 has not yet been referenced specifically in any publications.

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