SIVA overexpression 293T裂解液(全细胞) (ab94199)

概述

  • 产品名称SIVA overexpression 293T裂解液(全细胞)
    参阅全部 SIVA 细胞裂解液
  • 描述
    SIVA overexpression裂解液
  • 常规说明ab94199 is a 293T cell transfected lysate in which Human SIVA has been transiently over-expressed using a pCMV-SIVA plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis. Tissue specificity: Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon. PTM: Phosphorylated by ABL2/ARG in response to oxidative stress.

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应用

Our Abpromise guarantee covers the use of ab94199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

SIVA overexpression 293T lysate (whole cell) 图像

  • ab94199 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SIVA antibody (ab67620) at 1/500 dilution

    Lane 1 : SIVA overexpression 293T lysate (whole cell) (ab94199)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SIVA overexpression 293T lysate (whole cell) (ab94199)参考文献

ab94199 has not yet been referenced specifically in any publications.

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