Anti-SIV1 Nef抗体[17] (ab42361)

概述

  • 产品名称Anti-SIV1 Nef抗体[17]
  • 描述
    小鼠单克隆抗体[17] to SIV1 Nef
  • 特异性Reacts with SIV of mac251 strain.
  • 经测试应用适用于: ELISA, WB, Radioimmunoprecipitation, ICC/IFmore details
  • 种属反应性
    Simian Immunodeficiency Virus 1.
  • 免疫原

    Recombinant Nef protein of SIV1 mac251 strain.

  • 表位Mapped to amino acids 71-80 (PAEEREKLAY).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified by affinity chromatography.
  • 克隆单克隆
  • 克隆编号17
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/1000.
Radioimmunoprecipitation 1/5000.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性The Nef proteins of HIV-1 and SIV are 27- to 32 -kDa myristoylated proteins that are found in both the membranes and cytosol of infected host cells. While not strictly required for viral infection and replication, attenuation of Nef expression in either HIV-1 or SIV is associated with markedly delayed progression of disease. Simian immunodeficiency virus (SIV) and HIV-1 Nef proteins are related regulatory proteins that share several functions, including the ability to downregulate class I major histocompatibility complex (MHC) and CD4 expression on the cell surface and to alter T-cell-receptor-initiated signal transduction in T cells. Nef is thus probably involved in viral immune evasion mechanisms.
  • 细胞定位Cell Membrane, Lipid-anchor.
  • 别名
    • 3'ORF antibody
    • F protein antibody
    • Negative factor antibody

Anti-SIV1 Nef antibody [17] (ab42361)参考文献

ab42361 has not yet been referenced specifically in any publications.

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (PBMC)
Loading amount 25 µg
Specification PBMC
Treatment SIV-infected
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Karppiah Muthumani

Verified customer

提交于 Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"