Anti-SIV1 Nef抗体[17] (ab42361)

概述

  • 产品名称
    Anti-SIV1 Nef抗体[17]
  • 描述
    小鼠单克隆抗体[17] to SIV1 Nef
  • 特异性
    Reacts with SIV of mac251 strain.
  • 经测试应用
    适用于: ELISA, WB, Radioimmunoprecipitation, ICC/IFmore details
  • 种属反应性
    Simian Immunodeficiency Virus 1.
  • 免疫原

    Recombinant Nef protein of SIV1 mac251 strain.

  • 表位
    Mapped to amino acids 71-80 (PAEEREKLAY).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified by affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    17
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/1000.
Radioimmunoprecipitation 1/5000.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性
    The Nef proteins of HIV-1 and SIV are 27- to 32 -kDa myristoylated proteins that are found in both the membranes and cytosol of infected host cells. While not strictly required for viral infection and replication, attenuation of Nef expression in either HIV-1 or SIV is associated with markedly delayed progression of disease. Simian immunodeficiency virus (SIV) and HIV-1 Nef proteins are related regulatory proteins that share several functions, including the ability to downregulate class I major histocompatibility complex (MHC) and CD4 expression on the cell surface and to alter T-cell-receptor-initiated signal transduction in T cells. Nef is thus probably involved in viral immune evasion mechanisms.
  • 细胞定位
    Cell Membrane, Lipid-anchor.
  • 别名
    • 3'ORF antibody
    • F protein antibody
    • Negative factor antibody

Anti-SIV1 Nef antibody [17] (ab42361)参考文献

ab42361 has not yet been referenced specifically in any publications.

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For ab42361: DISCOUNT CODE: ***

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Expiration date: August 5th, 2012

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Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (PBMC)
Loading amount
25 µg
Specification
PBMC
Treatment
SIV-infected
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Karppiah Muthumani

Verified customer

提交于 Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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