Anti-SIT抗体[SIT-01] (ab9528)

概述

  • 产品名称Anti-SIT抗体[SIT-01]
    参阅全部 SIT 一抗
  • 描述
    小鼠单克隆抗体[SIT-01] to SIT
  • 特异性Human SHP2-interacting transmembrane adapter protein (SIT).
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant intracellular fragment (Human).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein-A affinity chromatography.
  • 克隆单克隆
  • 克隆编号SIT-01
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
  • 组织特异性Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.
  • 翻译后修饰Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • gp30/40 antibody
    • SHP intracting transmembrane adapter protein antibody
    • SHP2-interacting transmembrane adapter protein antibody
    • Signaling threshold-regulating transmembrane adapter 1 antibody
    • SIT antibody
    • Sit1 antibody
    • SIT1_HUMAN antibody
    • Suppression inducing transmembrane adapter 1 antibody
    • Suppression-inducing transmembrane adapter 1 antibody
    see all

Anti-SIT antibody [SIT-01] 图像

  • IHC image of ab9528 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SIT antibody [SIT-01] (ab9528)参考文献

ab9528 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"