概述

  • 产品名称Anti-SIRT7抗体
    参阅全部 SIRT7 一抗
  • 描述
    兔多克隆抗体to SIRT7
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SIRT7.

  • 阳性对照
    • This antibody gave a positive signal in Human Kidney tissue lysate and human breast adenocarcinoma FFPE tissue sections

性能

应用

Our Abpromise guarantee covers the use of ab105038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
  • 序列相似性Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻译后修饰Phosphorylated during mitosis.
  • 细胞定位Cytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NAD dependent deacetylase sirtuin 7 antibody
    • NAD dependent protein deacetylase sirtuin 7 antibody
    • NAD-dependent protein deacetylase sirtuin-7 antibody
    • Regulatory protein SIR2 homolog 7 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody
    • SIR2 L7 antibody
    • SIR2 like protein 7 antibody
    • Sir2 related protein type 7 antibody
    • SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody
    • SIR2-like protein 7 antibody
    • SIR2L 7 antibody
    • SIR2L7 antibody
    • SIR7_HUMAN antibody
    • SIRT 7 antibody
    • SIRT7 antibody
    • Sirtuin 7 antibody
    • Sirtuin type 7 antibody
    • Sirtuin7 antibody
    see all

Anti-SIRT7 antibody 图像

  • Anti-SIRT7 antibody (ab105038) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 55 kDa,70 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • All lanes : Anti-SIRT7 antibody (ab105038) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203) with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 55 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • IHC image of SIRT3 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105038, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SIRT7 antibody (ab105038)参考文献

ab105038 has not yet been referenced specifically in any publications.

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