Anti-SIRT6抗体- ChIP Grade (ab62739)

概述

  • 产品名称
    Anti-SIRT6抗体- ChIP Grade
    参阅全部 SIRT6 一抗
  • 描述
    兔多克隆抗体to SIRT6 - ChIP Grade
  • 经测试应用
    适用于: WB, ICC/IF, IHC-P, CHIPseq, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    GLPEIFDPPEELERK

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 19-33 of Human SIRT6

  • 阳性对照
    • Extracts of human U87 cells, extracts of mouse 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab62739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200.
CHIPseq Use at an assay dependent concentration. PubMed: 22196736
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • 序列相似性
    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 细胞定位
    Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody - ChIP Grade 图像

  • ChIP analysis using ab62739 binding SIRT6 in mouse primary hepatoctyes. Cells were cross-linked for 8 minutes with 1% formaldehyde. Samples were incubated with Anti-SIRT6 antibody - ChIP Grade (ab62739) (1/200) for 20 hours at 4°C. Protein binding was detected using real-time PCR.

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  • ab62739 staining SIRT6 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

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  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SIRT6 with ab62739. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Anti-SIRT6 antibody - ChIP Grade (ab62739). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Lane 1 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 1 µg/ml
    Lanes 3 - 4 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 2 µg/ml

    Lane 1 : Mouse 3T3 whole cell extract
    Lane 2 : Mouse 3T3 whole cell extract
    Lane 3 : Mouse 3T3 whole cell extract
    Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG,
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • Immunocytochemistry/ Immunofluorescence analysis of mouse primary hepatocytes labeling SIRT6 with ab62739 at 1/200 dilution. The cells were fixed with paraformaldehyde, followed by blocking with 3% BSA for 2 hours at 20°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 488 secondary antibody was used at 1/10000 dilution.

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Anti-SIRT6 antibody - ChIP Grade (ab62739)参考文献

This product has been referenced in:
  • Zhang ZQ  et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep 6:23912 (2016). ChIP, WB . Read more (PubMed: 27045575) »
  • Kim TH  et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta 1850:401-10 (2015). Read more (PubMed: 25445714) »

See all 16 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
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提交于 Jul 17 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions
Reduced Denaturing (gradient)
Loading amount
10 µg
Specification
Hepatocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Sep 06 2016

Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Negative control
Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
H3k9ac
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提交于 Dec 04 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 29°C
Sample
Mouse Cell (GL261-mouse glioma)
Specification
GL261-mouse glioma
Permeabilization
Yes - 0.3% TritonX-100 in 1X TBS
Fixative
Paraformaldehyde
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提交于 Dec 03 2014

Application
Western blot
Loading amount
240000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (Primary Neural cells)
Specification
Primary Neural cells
Treatment
Supplemented with and without serum in the culture media.
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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提交于 Sep 16 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 07 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Permeabilization
No
Fixative
Paraformaldehyde
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提交于 Jun 28 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Cell lysate - whole cell (55.1C cell)
Specification
55.1C cell
Total protein in input
30 µg
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提交于 Mar 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (primary hepatocytes)
Specification
primary hepatocytes
Permeabilization
No
Fixative
Paraformaldehyde
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提交于 Jan 23 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
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提交于 Oct 29 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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