The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Use at a concentration of 0.5 - 1.0 ug/ml, using whole extracts of human U87 cells and at a concentration of 2.5 - 5.0 ug/ml, using human HeLa cells. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Use a concentration of 2.5 - 5 µg/ml.
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Belongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
Immunocytochemistry/ Immunofluorescence analysis of human HeLa cells fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. SIRT6 was labeled with 5 µg/mL ab62738 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A) or with DAPI (B).
Western blot - SIRT6 antibody (ab62738)
Lane 1 : Anti-SIRT6 antibody (ab62738) at 0.5 µg/ml Lane 2 : Anti-SIRT6 antibody (ab62738) at 1 µg/ml Lanes 3 - 4 : Anti-SIRT6 antibody (ab62738) at 2 µg/ml
Lane 1 : Human U87 whole cell extract Lane 2 : Human U87 whole cell extract Lane 3 : Human U87 whole cell extract Lane 4 : Human U87 whole cell extract with immunizing peptide
Secondary Goat Anti-Rabbit IgG Developed using the ECL technique
Predicted band size : 39 kDa Observed band size : 39 kDa