概述

  • 产品名称Anti-SIRT6抗体
    参阅全部 SIRT6 一抗
  • 描述
    兔多克隆抗体to SIRT6
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    SPKRERPTSPAPHRPPKRV

    conjugated to KLH by a Cysteine residue linker, corresponding to C terminal amino acids 330-348 of Human SIRT6

  • 阳性对照
    • Extracts from human U87 cells, HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Use at a concentration of 0.5 - 1.0 ug/ml, using whole extracts of human U87 cells and at a concentration of 2.5 - 5.0 ug/ml, using human HeLa cells. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 2.5 - 5 µg/ml.

靶标

  • 功能NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • 序列相似性Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 细胞定位Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody 图像

  • Immunocytochemistry/ Immunofluorescence analysis of human HeLa cells fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. SIRT6 was labeled with 5 µg/mL ab62738 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A) or with DAPI (B).

  • Lane 1 : Anti-SIRT6 antibody (ab62738) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody (ab62738) at 1 µg/ml
    Lanes 3 - 4 : Anti-SIRT6 antibody (ab62738) at 2 µg/ml

    Lane 1 : Human U87 whole cell extract
    Lane 2 : Human U87 whole cell extract
    Lane 3 : Human U87 whole cell extract
    Lane 4 : Human U87 whole cell extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-SIRT6 antibody (ab62738)参考文献

ab62738 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (smooth muscle cells)
Loading amount 10 µg
Specification smooth muscle cells
Treatment senescence
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung)
Loading amount 100000 cells
Specification Lung
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"