概述

  • 产品名称Anti-SIRT6抗体
    参阅全部 SIRT6 一抗
  • 描述
    兔多克隆抗体to SIRT6
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Chicken, Pig, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse SIRT6.

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse tissue lysates: Thymus; Heart; Brain; Forebrain.

性能

应用

Our Abpromise guarantee covers the use of ab124293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • 序列相似性Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 细胞定位Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody 图像

  • All lanes : Anti-SIRT6 antibody (ab124293) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Forebrain (Mouse) Tissue Lysate
    Lane 4 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 51 kDa,71 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-SIRT6 antibody (ab124293)参考文献

ab124293 has not yet been referenced specifically in any publications.

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