• 产品名称Anti-SIRT4抗体
    参阅全部 SIRT4 一抗
  • 描述
    兔多克隆抗体to SIRT4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide designed within residues: SPPLDPEKVK ELQRFITLSK RLLVMTGAGI STESGIPDYR SEKVGLYART, corresponding to N terminal amino acids 36 - 85 of Human SIRT4 (NP_036372).

  • 阳性对照
    • Human fetal small intestine lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas.


Our Abpromise guarantee covers the use of ab90485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.


  • 功能Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion.
  • 组织特异性Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
  • 序列相似性Belongs to the sirtuin family. Class II subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC130046 antibody
    • MGC130047 antibody
    • MGC57437 antibody
    • NAD dependent deacetylase sirtuin 4 antibody
    • NAD dependent protein deacetylase sirtuin 4 antibody
    • NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody
    • NAD-dependent protein deacetylase sirtuin-4 antibody
    • NAD-dependent protein lipoamidase sirtuin-4, mitochondrial antibody
    • Regulatory protein SIR2 homolog 4 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Sir 2 like 4 antibody
    • SIR 2 like protein 4 antibody
    • Sir2 like 4 antibody
    • SIR2 like protein 4 antibody
    • SIR2-like protein 4 antibody
    • SIR2L 4 antibody
    • SIR2L4 antibody
    • SIR4_HUMAN antibody
    • SIRT 4 antibody
    • Sirt4 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 4 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody
    • Sirtuin 4 antibody
    • Sirtuin type 4 antibody
    • Sirtuin-4 antibody
    • Sirtuin4 antibody
    see all

Anti-SIRT4 antibody 图像

  • Anti-SIRT4 antibody (ab90485) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
  • IHC image of SIRT4 staining in Human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90485, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SIRT4 antibody (ab90485)参考文献

ab90485 has not yet been referenced specifically in any publications.

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Thank you very much for confirming these details. I would be pleased to replace the antibody with an Sirt4 antibody. If the customer is interested in an antibody for IHC-P, I agree that ab10140, ab105039 and ab115517 are suitable. As mentioned on t...

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