概述

  • 产品名称Anti-SIRT3抗体
    参阅全部 SIRT3 一抗
  • 描述
    兔多克隆抗体to SIRT3
  • 特异性Strongly expressed in liver and kidney. Weakly expressed in lung.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Cynomolgus Monkey, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Mouse SIRT3 aa 317-330 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    ELLDLMQRERGKLD


    Database link: Q8R104
    (Peptide available as ab176315)

  • 阳性对照
    • WB: Mouse brain tissue lysate. CHO whole cell lysate. ICC/IF: HepG2 cells. IHC-P: Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab86671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 36.6 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻译后修饰Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSIRT 3 antibody
    • hSIRT3 antibody
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
    • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
    • Regulatory protein SIR2 homolog 3 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
    • Sir 2 like 3 antibody
    • SIR 2 like protein 3 antibody
    • SIR 3 antibody
    • SIR2 L3 antibody
    • Sir2 like 3 antibody
    • SIR2 like protein 3 antibody
    • SIR2-like protein 3 antibody
    • SIR2L3 antibody
    • SIR3_HUMAN antibody
    • SIRT 3 antibody
    • SIRT3 antibody
    • Sirtuin 3 antibody
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
    • Sirtuin type 3 antibody
    • Sirtuin3 antibody
    see all

Anti-SIRT3 antibody 图像

  • Anti-SIRT3 antibody (ab86671) at 1/1000 dilution + Mouse brain tissue lysate at 35 µg/ml

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 36.6 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SIRT3 antibody (ab86671) at 1/1000 dilution + CHO whole cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 36.6 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinomaa tissue labelling SIRT3 with ab86671. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab86671 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 rabbit anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SIRT3 antibody (ab86671)参考文献

This product has been referenced in:
  • Cornelius N  et al. Cellular consequences of oxidative stress in riboflavin responsive multiple acyl-CoA dehydrogenation deficiency patient fibroblasts. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24698980) »
  • Hindle AG  et al. Metabolic changes associated with the long winter fast dominate the liver proteome in 13-lined ground squirrels. Physiol Genomics 46:348-61 (2014). Read more (PubMed: 24642758) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Specification Peripheral Blood Mononuclear Cell
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Daniel Tyrrell

Verified customer

提交于 Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 25 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2012

Thank you for contacting us.

As we discussed over the phone, I think the band you detect at 39 kDa is the correct band.

In order to reduce the background bands, I would suggest the following:
1) use less priamry antibody (e.g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"