概述

性能

应用

Our Abpromise guarantee covers the use of ab75434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).

靶标

  • 功能
    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻译后修饰
    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
    • 别名
      • hSIRT 3 antibody
      • hSIRT3 antibody
      • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
      • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
      • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
      • Regulatory protein SIR2 homolog 3 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
      • Sir 2 like 3 antibody
      • SIR 2 like protein 3 antibody
      • SIR 3 antibody
      • SIR2 L3 antibody
      • Sir2 like 3 antibody
      • SIR2 like protein 3 antibody
      • SIR2-like protein 3 antibody
      • SIR2L3 antibody
      • SIR3_HUMAN antibody
      • SIRT 3 antibody
      • SIRT3 antibody
      • Sirtuin 3 antibody
      • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
      • Sirtuin type 3 antibody
      • Sirtuin3 antibody
      see all

    图片

    • Western blot - Anti-SIRT3 antibody (ab75434)
      Western blot - Anti-SIRT3 antibody (ab75434)
      Anti-SIRT3 antibody (ab75434) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 42 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 16 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      The band observed at 42 kDa could potentially be a cleaved form of SIRT3 due to the presence of a transit peptide.

    实验方案

    文献

    This product has been referenced in:
    • Takakura K  et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions. Heliyon 3:e00462 (2017). WB ; Rat . Read more (PubMed: 29264419) »
    • Li S  et al. p53-Induced growth arrest is regulated by the mitochondrial SirT3 deacetylase. PLoS One 5:e10486 (2010). WB ; Human . Read more (PubMed: 20463968) »

    See all 2 Publications for this product

    发表研究结果有使用 ab75434？请让我们知道，以便我们可以引用本数据表中的参考文章。

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