概述

  • 产品名称Anti-SIRT3抗体
    参阅全部 SIRT3 一抗
  • 描述
    兔多克隆抗体to SIRT3
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SIRT3.

    (Peptide available as ab45065.)

  • 阳性对照
    • ab40963 gave a positive signal in Human Liver Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab40963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

靶标

  • 功能NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻译后修饰Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSIRT 3 antibody
    • hSIRT3 antibody
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
    • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
    • Regulatory protein SIR2 homolog 3 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
    • Sir 2 like 3 antibody
    • SIR 2 like protein 3 antibody
    • SIR 3 antibody
    • SIR2 L3 antibody
    • Sir2 like 3 antibody
    • SIR2 like protein 3 antibody
    • SIR2-like protein 3 antibody
    • SIR2L3 antibody
    • SIR3_HUMAN antibody
    • SIRT 3 antibody
    • SIRT3 antibody
    • Sirtuin 3 antibody
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
    • Sirtuin type 3 antibody
    • Sirtuin3 antibody
    see all

Anti-SIRT3 antibody 图像

  • Anti-SIRT3 antibody (ab40963) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,36 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab40963 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40963, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SIRT3 antibody (ab40963)参考文献

ab40963 has not yet been referenced specifically in any publications.

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