概述

  • 产品名称Anti-SIRT2抗体
    参阅全部 SIRT2 一抗
  • 描述
    兔多克隆抗体to SIRT2
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cynomolgus Monkey, Marmoset (common), Orangutan
  • 免疫原

    Synthetic peptide:

    LEDLVRREHA NI

    conjugated to KLH, corresponding to amino acids 341-352 of Mouse SIR2/ SIRT2

  • 阳性对照
    • Whole extracts of mouse and rat brain. Extract of COS7 cells expressing recombinant human SIRT2 protein. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab67299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 , 38 kDa.
IP Use at an assay dependent dilution. Use at 2 - 5 µg.
ICC/IF Use a concentration of 5 - 10 µg/ml.

靶标

  • 功能NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 组织特异性Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 序列相似性Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 发展阶段Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻译后修饰Phosphorylated at the G2/M transition of the cell cycle.
  • 细胞定位Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody 图像

  • ab67299 staining SIRT2 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for  60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An  Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation  of rSCs with RSV led to an increase of SIRT2 expression in densitometry analysis.

Anti-SIRT2 antibody (ab67299)参考文献

This product has been referenced in:
  • Budayeva HG & Cristea IM Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking. Mol Cell Proteomics 15:3107-3125 (2016). Read more (PubMed: 27503897) »
  • Stettner M  et al. Promoting myelination in an in vitro mouse model of the peripheral nerve system: the effect of wine ingredients. PLoS One 8:e66079 (2013). ICC/IF ; Rat . Read more (PubMed: 23762469) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 240000 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Primary Neural cells)
Specification Primary Neural cells
Treatment Supplemented with and without serum in the culture media.
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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提交于 Sep 12 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate with 0.05% Tween 20 pH 6
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% triton X 100
Fixative Paraformaldehyde
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提交于 Nov 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hypothalamus)
Loading amount 50 µg
Specification Hypothalamus
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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提交于 Feb 15 2013

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Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse an...

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