概述

  • 产品名称Anti-SIRT2抗体
    参阅全部 SIRT2 一抗
  • 描述
    兔多克隆抗体to SIRT2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide: MAEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS, corresponding to a region within N terminal amino acids 1-50 of Human SIRT2

  • 阳性对照
    • Fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab32829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 组织特异性Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 序列相似性Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 发展阶段Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻译后修饰Phosphorylated at the G2/M transition of the cell cycle.
  • 细胞定位Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody 图像

  • Anti-SIRT2 antibody (ab32829) at 0.25 µg/ml + Fetal brain lysate (10ug).

    Secondary
    HRP conjugated anti Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-SIRT2 antibody (ab32829)参考文献

ab32829 has not yet been referenced specifically in any publications.

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