概述

  • 产品名称Anti-SIRT2抗体
    参阅全部 SIRT2 一抗
  • 描述
    兔多克隆抗体to SIRT2
  • 特异性SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms.
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human SIRT2 aa 377-389.
    Sequence:

    DEARTTEREKPQ

  • 阳性对照
    • SAOS2 transfected cell lysates, human chondrocyte lysates.
  • 常规说明


    The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.

性能

应用

Our Abpromise guarantee covers the use of ab19388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
ICC 1/400.

靶标

  • 功能NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 组织特异性Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 序列相似性Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 发展阶段Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻译后修饰Phosphorylated at the G2/M transition of the cell cycle.
  • 细胞定位Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody 图像

  • Anti-SIRT2 antibody (ab19388) at 1/1000 dilution

    Predicted band size : 43 kDa
  • Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.

Anti-SIRT2 antibody (ab19388)参考文献

This product has been referenced in:
  • Lara E  et al. Salermide, a Sirtuin inhibitor with a strong cancer-specific proapoptotic effect. Oncogene 28:781-91 (2009). WB ; Human . Read more (PubMed: 19060927) »
  • Lovato L  et al. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics : (2008). WB ; Human . Read more (PubMed: 18676363) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply, the refund will be arranged soon by our accounts department. I'm afraid we do not have the immunogen peptide in our catalogue nor can we source it for you as ab19388 is not made in house, I'm very sorry I cannot help you more,

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I'm sorry to hear you are experiencing problems with ab19388. Many thanks for providing the image of your blot and your detailed protocol, it is very useful for me to understand your problem. I would like to recommend some changes to your protocol ...

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Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not h...

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Thank you for your email, and I'm replying on behalf of Sarah who is currently away from the office. I'm sorry to hear that ab19388 is still giving you difficulty and I can certainly arrange for a free of charge replacement vial to be sent to you. ...

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Thank you for contacting us for technical support and for taking the time to provide all your protocol details, this was really useful to understand your problem. I suspect the problem is due to the blocking agent present in your antibody dilution b...

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