Anti-SIRT2抗体(ab19388)
Key features and details
- Rabbit polyclonal to SIRT2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SIRT2抗体
参阅全部 SIRT2 一抗 -
描述
兔多克隆抗体to SIRT2 -
宿主
Rabbit -
特异性
SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human SIRT2 aa 377-389.
Sequence:DEARTTEREKPQ
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阳性对照
- ICC/IF: SAOS2 transfected cell lysates WB: HeLa and MCF7 whole cell lysate; Mouse brain and liver tissue lysates IHC-P: Human heart tissue
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常规说明
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
Primary antibody说明
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19388于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100.
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|
WB |
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
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ICC/IF |
1/400.
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说明 |
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IHC-P
1/100. |
WB
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
1/400. |
靶标
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功能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
组织特异性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
序列相似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
发展阶段
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻译后修饰
Phosphorylated at the G2/M transition of the cell cycle. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- Unigene: 466693 Human
- Unigene: 272443 Mouse
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别名
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
图片
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All lanes : Anti-SIRT2 antibody (ab19388) at 1/250 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, HRP conjugate at 1/5000 dilution
Predicted band size: 43 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SIRT2 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab19388 diluted in 3% BSA-PBS at a dilution of 1/100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab19388 被引用在 3 文献中.
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Lara E et al. Salermide, a Sirtuin inhibitor with a strong cancer-specific proapoptotic effect. Oncogene 28:781-91 (2009). WB ; Human . PubMed: 19060927
- Lovato L et al. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics : (2008). WB ; Human . PubMed: 18676363