SIRT2 Activity Assay试剂盒(Fluorometric) (ab156066)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
概述
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产品名称
SIRT2 Activity Assay试剂盒(Fluorometric)
参阅全部 SIRT2 试剂盒 -
检测方法
Fluorescent -
检测类型
Enzyme activity -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
Abcam’s SIRT2 Activity Assay Kit (Fluorometric) (ab156066) detects deacetylase activity of recombinant SIRT2.
Primarily, Abcam’s SIRT2 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT2.
2. Detecting the effects of pharmacological agents on SIRT2.
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说明
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 100 tests Developer 1 x 500µl Fluoro-Deacetylated Peptide (0.08 mM) 1 x 100µl Fluoro-Substrate Peptide (0.08 mM) 1 x 500µl NAD (8 mM) 1 x 500µl Recombinant SIRT2 1 x 500µl SIRT Assay Buffer 2 x 1ml Stop Solution 2 x 1ml -
研究领域
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功能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
组织特异性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
序列相似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
发展阶段
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻译后修饰
Phosphorylated at the G2/M transition of the cell cycle. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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别名
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all -
数据库链接
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Entrez Gene: 361532 Rat
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- SwissProt: Q5RJQ4 Rat
- Unigene: 466693 Human
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab156066 被引用在 4 文献中.
- Xu D et al. SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation. Int J Mol Sci 20:N/A (2019). PubMed: 30889926
- Wang X et al. Cysteine thiol oxidation on SIRT2 regulates inflammation in obese mice with sepsis. Inflammation 42:156-169 (2019). PubMed: 30203196
- Li J et al. Insight into the Mechanism of Intramolecular Inhibition of the Catalytic Activity of Sirtuin 2 (SIRT2). PLoS One 10:e0139095 (2015). Functional Studies . PubMed: 26407304
- Ponnusamy M et al. Blocking sirtuin 1 and 2 inhibits renal interstitial fibroblast activation and attenuates renal interstitial fibrosis in obstructive nephropathy. J Pharmacol Exp Ther 350:243-56 (2014). PubMed: 24833701