为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Our Abpromise guarantee covers the use of ab110304 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|WB||Use a concentration of 0.125 µg/ml. Predicted molecular weight: 81 kDa.
Detects a band of approximately 110 kDa (110-121 kDa) which is likely to be due to post translational glycosylation. SIRT1 is known to bind to several other proteins, and the 121 kDa band could also be due to the presence of one of these complexes (ensure samples are adequately reduced and denatured).
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 0.5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
IHC image of SIRT1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110304, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
This image is courtesy of an anonymous Abreview
Blocked with 3% milk (TBS-tween) at 4C for 16 hours