SIRP alpha overexpression 293T裂解液(全细胞) (ab94139)

概述

  • 产品名称SIRP alpha overexpression 293T裂解液(全细胞)
  • 描述
    SIRP alpha overexpression裂解液
  • 常规说明ab94139 is a 293T cell transfected lysate in which Human SIRP alpha has been transiently over-expressed using a pCMV-SIRP alpha plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Tissue specificity: Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. Similarity: Contains 2 Ig-like C1-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. PTM: N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.

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应用

Our Abpromise guarantee covers the use of ab94139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

SIRP alpha overexpression 293T lysate (whole cell) 图像

  • ab94139 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SIRP alpha antibody (ab77061) at 1/500 dilution

    Lane 1 : SIRP alpha overexpression 293T lysate (whole cell) (ab94139)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SIRP alpha overexpression 293T lysate (whole cell) (ab94139)参考文献

ab94139 has not yet been referenced specifically in any publications.

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