概述

  • 产品名称Anti-SIPA1抗体
    参阅全部 SIPA1 一抗
  • 描述
    兔多克隆抗体to SIPA1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to a region between residue 992 to 1042 of human SIPA1 (NP_006738.3).

  • 阳性对照
    • HeLa cells, 293T and mouse NIH3T3 whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab85928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
    • 组织特异性Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
    • 序列相似性Contains 1 PDZ (DHR) domain.
      Contains 1 Rap-GAP domain.
    • 细胞定位Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GTPase activating protein Spa 1 antibody
      • GTPase-activating protein Spa-1 antibody
      • MGC102688 antibody
      • MGC17037 antibody
      • p130 SPA-1 antibody
      • p130 SPA1 antibody
      • Signal induced proliferation associated 1 antibody
      • Signal induced proliferation associated gene 1 antibody
      • Signal induced proliferation associated protein 1 antibody
      • Signal-induced proliferation-associated protein 1 antibody
      • SIPA 1 antibody
      • Sipa-1 antibody
      • Sipa1 antibody
      • SIPA1_HUMAN antibody
      • Spa1 antibody
      see all

    Anti-SIPA1 antibody 图像

    • Detection of SIPA1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 30 seconds.
    • All lanes : Anti-SIPA1 antibody (ab85928) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    Anti-SIPA1 antibody (ab85928)参考文献

    ab85928 has not yet been referenced specifically in any publications.

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