Anti-Spa-1抗体(ab85928)
Key features and details
- Rabbit polyclonal to Spa-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Spa-1抗体
参阅全部 Spa-1 一抗 -
描述
兔多克隆抗体to Spa-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Spa-1 aa 992-1042.
Database link: NP_006738.3 -
阳性对照
- HeLa cells, 293T and mouse NIH3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85928 was affinity purified using an epitope specific to Spa-1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 112 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 112 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. -
组织特异性
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord. -
序列相似性
Contains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain. -
细胞定位
Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region. - Information by UniProt
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数据库链接
- Entrez Gene: 6494 Human
- Entrez Gene: 20469 Mouse
- Omim: 602180 Human
- SwissProt: Q96FS4 Human
- SwissProt: P46062 Mouse
- Unigene: 530477 Human
- Unigene: 3072 Mouse
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别名
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
see all
图片
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Detection of Spa-1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
All lanes : Anti-Spa-1 antibody (ab85928) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85928 被引用在 1 文献中.
- Yao C et al. SIPA1 Enhances Aerobic Glycolysis Through HIF-2α Pathway to Promote Breast Cancer Metastasis. Front Cell Dev Biol 9:779169 (2021). PubMed: 35096814