Anti-SIPA1抗体(ab10162)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SIPA1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: ASKQLGSPTADLA, corresponding to C terminal amino acids 1030-1042 of Human SIPA1.

  • 阳性对照
    • Human lymph node and Jurkat.
  • 常规说明


    SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region.

性能

应用

Our Abpromise guarantee covers the use of ab10162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).Can be blocked with Human SIPA1 peptide (ab23193).

靶标

  • 功能
    GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
  • 组织特异性
    Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
  • 序列相似性
    Contains 1 PDZ (DHR) domain.
    Contains 1 Rap-GAP domain.
  • 细胞定位
    Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GTPase activating protein Spa 1 antibody
    • GTPase-activating protein Spa-1 antibody
    • MGC102688 antibody
    • MGC17037 antibody
    • p130 SPA-1 antibody
    • p130 SPA1 antibody
    • Signal induced proliferation associated 1 antibody
    • Signal induced proliferation associated gene 1 antibody
    • Signal induced proliferation associated protein 1 antibody
    • Signal-induced proliferation-associated protein 1 antibody
    • SIPA 1 antibody
    • Sipa-1 antibody
    • Sipa1 antibody
    • SIPA1_HUMAN antibody
    • Spa1 antibody
    see all

Anti-SIPA1 antibody 图像



  • Predicted band size : 130 kDa

    This image was submitted as part of a review by Kathy Till

实验方案

Anti-SIPA1 antibody (ab10162)参考文献

ab10162 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human B cells)
Loading amount
20 µg
Specification
Human B cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Kathy Till

Verified customer

提交于 Apr 19 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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