概述

  • 产品名称Anti-Sin3b抗体
    参阅全部 Sin3b 一抗
  • 描述
    兔多克隆抗体to Sin3b
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region of Human Sin3b (NP_056075).

  • 阳性对照
    • Fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab76231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 133 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 相关性Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Histone deacetylase complex subunit Sin3b antibody
    • Paired amphipathic helix protein Sin3b antibody
    • SIN3 homolog B transcription regulator antibody
    • SIN3 transcription regulator family member B antibody
    • SIN3 transcription regulator homolog B antibody
    • Transcriptional corepressor Sin3b antibody
    see all

Anti-Sin3b antibody 图像

  • Anti-Sin3b antibody (ab76231) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

Anti-Sin3b antibody (ab76231)参考文献

ab76231 has not yet been referenced specifically in any publications.

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