概述

  • 产品名称Anti-SIN1抗体
    参阅全部 SIN1 一抗
  • 描述
    兔多克隆抗体to SIN1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 522) of human SIN1 (NP_001006618.1).

  • 阳性对照
    • Whole cell lysate from Hela cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab71152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 30, 50, 65 kDa (predicted molecular weight: 59 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
  • 组织特异性Ubiquitously expressed, with highest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the SIN1 family.
  • 细胞定位Cell membrane. Cytoplasmic vesicle. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAPKAP 1 antibody
    • MAPKAP1 antibody
    • MEKK2 interacting protein 1 antibody
    • MGC2745 antibody
    • MIP 1 antibody
    • MIP1 antibody
    • Mitogen activated protein kinase associated protein 1 antibody
    • Mitogen-activated protein kinase 2-associated protein 1 antibody
    • mSIN1 antibody
    • OTTHUMP00000064207 antibody
    • Ras inhibitor MGC2745 antibody
    • SAPK interacting protein 1 antibody
    • SAPK-interacting protein 1 antibody
    • SIN 1 antibody
    • SIN1_HUMAN antibody
    • SIN1b antibody
    • SIN1g antibody
    • Stress activated map kinase interacting protein 1 antibody
    • Stress activated protein kinase interacting 1 antibody
    • Stress-activated map kinase-interacting protein 1 antibody
    • Target of rapamycin complex 2 subunit MAPKAP1 antibody
    • TORC2 subunit MAPKAP1 antibody
    see all

Anti-SIN1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SIN1 with ab71152 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SIN1 antibody (ab71152) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg


    Predicted band size : 59 kDa
    Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of SIN1
    Lane 1: ab71152 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG IP.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed with 1 µg/ml ab71152.
    Chemiluminescence with an exposure time of 30 seconds.

Anti-SIN1 antibody (ab71152)参考文献

This product has been referenced in:
  • González-Barriga A  et al. Design and analysis of effects of triplet repeat oligonucleotides in cell models for myotonic dystrophy. Mol Ther Nucleic Acids 2:e81 (2013). WB ; Mouse, Human . Read more (PubMed: 23511335) »
  • Joha S  et al. GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene : (2011). Read more (PubMed: 21804606) »

See all 2 Publications for this product

Product Wall

rply. Please find here below the discount code for testing the Anti-SIN1 antibody (ab71152) on Zebra fish.

I have checked also for our other antibodies against Rictor.

We do have 8 antibodies tested in Western blot against Rictor, p...

Read More

Thank you for your inquiry and your interest in our antibodies!

I have contacted the laboratory to find out whether the immunogen sequence is similar to the Danio rerio sequence. The immunogen sequence is proprietary information, however the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"