概述

  • 产品名称Anti-Silencer of Death Domain抗体
    参阅全部 Silencer of Death Domain 一抗
  • 描述
    兔多克隆抗体to Silencer of Death Domain
  • 特异性Human, Mouse, Rat.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    SALRRSGYGPSDGPSC

    , corresponding to amino acids 2-16 of Human SODD.

  • 阳性对照
    • HeLa whole cell lysate or THP-1 whole cell lysate.
  • 常规说明Silencer of Death Domains.


    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明SODD Antibody is Antibody is DEAE purified.
  • Primary antibody说明Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab2048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa.Can be blocked with Silencer of Death Domain peptide (ab8382).

靶标

Anti-Silencer of Death Domain antibody 图像

  • All lanes : Anti-Silencer of Death Domain antibody (ab2048) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with Silencer of Death Domain peptide (ab8382)
    Lane 4 : THP-1 whole cell lysate with Silencer of Death Domain peptide (ab8382)


    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab2048 at 5µg/ml staining Silencer of Death Domain in Hela cells by ICC/IF 

  • Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.

Anti-Silencer of Death Domain antibody (ab2048)参考文献

This product has been referenced in:
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . Read more (PubMed: 23341456) »

See 1 Publication for this product

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